Bob Hoskins
Eddie Valiant
Christopher Lloyd
Judge Doom
Joanna Cassidy
Dolores
Charles Fleischer
Roger Rabbit / Benny The Cab / Greasy / Psycho (voice)
Kathleen Turner
Jessica Rabbit (voice)
Stubby Kaye
Marvin Acme
Alan Tilvern
R.K. Maroon
Richard LeParmentier
Lt. Santino
Lou Hirsch
Baby Herman (voice)
Betsy Brantley
Jessica's Performance Model
Joel Silver
Raoul
Richard Ridings
Angelo
Mike Edmonds
Stretch
Morgan Deare
Editor / Gorilla (voice)
Billy J. Mitchell
Forensic #2
Ed Herlihy
Newscaster
James O'Connell
Conductor
April Winchell
Mrs. Herman (voice)
Mae Questel
Betty Boop (voice)
Mel Blanc
Bugs Bunny / Daffy Duck / Tweety Bird (voice)
Tony Anselmo
Donald Duck (voice)
Joe Alaskey
Yosemite Sam / Foghorn Leghorn (voice)
David L. Lander
Smart Ass (voice)
Fred Newman
Stupid (voice)
June Foray
Wheezy / Lena Hyena (voice)
Russi Taylor
Birds / Minnie Mouse (voice)
Richard Williams
Droopy (voice)
Wayne Allwine
Mickey Mouse (voice)
Pat Buttram
Bullet #1
Jim Cummings
Bullet #2
Jim Gallant
Bullet #3
Frank Sinatra
Singing Sword (voice) (archive sound)
Tony Pope
Goofy / Big Bad Wolf (voice)
Cherry Davis
Woody Woodpecker
Jack Angel
Additional Voices (voice) (uncredited)
Corey Burton
Judge Doom (high-pitched voice) (voice) (uncredited)
Nancy Cartwright
Dipped Shoe (voice) (uncredited)
Amy Irving
Jessica Rabbit (singing voice) (uncredited)
Mickie McGowan
Mooing Cow / Flying Witch (voice) (uncredited)
Frank Welker
Dumbo (voice) (uncredited)
Tina Simmons
Ink and Paint Club Patron (uncredited)
Paul Springer
Augie
Edwin Craig
Arthritic Cowboy
Lindsay Holiday
Soldier
Danny Capri
Kid #1
Christopher Hollosy
Kid #2
Jean-Paul Sipla
Kid #3
Laura Frances
Blonde Starlet
Joel Cutrara
Forensic #1
Eric B. Sindon
Mailman
Eugene Gutierrez
Teddy Valiant
Mary T. Radford
Hippo (voice)
Les Perkins
Mr. Toad (voice)
Peter Westy
Pinocchio (voice)
Jeff Arbaugh
Mime (uncredited)